The colonial style building, constructed in 1882, displays stuffed animal specimens and items of the Hokkaido Colonization Commission (1869-1882) and of Japanese Antarctic Research Expedition in 1950s.
　Stuffed specimen collections include animals native to Hokkaido such as Brown bear and Yezo sika deer, extinct Japanese river otter, and the only two known specimens of extinct Ezo wolf. Bird specimens collected by an English naturalist Thomas Blakiston, based on which he discussed a faunal boundary between Hokkaido and southern islands of Japan, are displayed with animal specimens of various kinds representing both the faunas.
　Collections related to the Hokkaido Colonization Commission include mineralogical and petrological specimens collected by an American mining engineer and foreign advisor Benjamin Lyman, and canned foods made by a government canning factory.
　The stuffed body of a Sakhalin husky named Taro is popular for its exploits as a sled dog in the first Japanese Antarctic Research Expedition in 1956, in which faculty members of Hokkaido University participated.

　In the garden, there are three remains of pit dwellings of Satsumon culture (the 7-13th century), indicating that there was a village in the garden located in this fertile land with natural springs. Satsumon-style earthenwares were also found in the garden, some of which are displayed in our museum.

　Prof. Miyabe Kingo (1860-1951) was the second-generation student of Sapporo Agricultural College (current Hokkaido University) and served as the first director of the botanic garden. The building, built in 1901 and designated as a Registered Tangible Cultural Property of Japan in 2000, displays items related to Prof. Miyabe, including his notebooks during college, field notes recording plant collections, microscope, etc.